Molecular Mechanisms of Inorganic-Phosphate Release from the Core and Barbed End of Actin Filaments
Published in Nature Structural & Molecular Biology, 2023
Recommended citation: W. Oosterheert*, F.E.C. Blanc*, A. Roy, A. Belyy, O. Hofnagel, G. Hummer, P. Bieling, S. Raunser (2023). "Molecular Mechanisms of Inorganic-Phosphate Release from the Core and Barbed End of Actin Filaments." Nature Structural & Molecular Biology. https://doi.org/10.1038/s41594-023-01101-9
We combine cryo-EM, enhanced sampling MD simulations, machine learning, and in vivo and in vitro reconstitution to reveal the mechanism and pathway for Pi release from F-actin, both from filament interior and barbed end.